Stemcelldoc's Weblog

August 5, 2012

PRP Concentrate Lecture at American Academy of Orthopedic Medicine

Today I discussed the Biologic Response of Human Mesenchymal Stem Cells to various PRP (platelet rich plasma) preps at the AAOM conference.  PRP has received signficant attention in the treatment of musculoskeletal and orthopedic injuries. At the Centeno-Schultz Clinic advanced platelet rich therapies are available for the treatment of common orthopedic conditions.

A previous blog (dirty little machine) outlined the problems associated with standard bedside centrifuge systems.  Specifically the PRP is contaminated with RBC’s which can led to a number of issues including inflammation as evidenced by signficant postprocedure swelling.  In contrast, Regenexx PL and SCP both of which are created by a cell biologist are free of RBC’s.

Are there other differences?


The is a striking difference in the ability of different PRP concentrates to grow human mesenchymal stem cells.  PRP concentrates with RBC’s were compared with Regenexx PL and SCP  in their ability to grow human mesenchymal stem cells in culture.  Both Regenexx PL and SCP are free of RBC contamination.

The results.

There was no growth of  stem cells with bedside centrifuge PRP preps that contained  RBC’s.  In contrast Regenexx PL and SCP demonstrated signficant stem cell growth. 

The picture tells the story.

After 6 days in culture there was mesenchymal stem cell growth as reflected by the presence of  colonies (MCU) in PRP preps that contained no RBC’s.  This is seen in top and bottom pictures on left.  In contrast there was no growth the PRP prep that contained RBCs as seen in upper right hand picture.

The presence of RBC’s in platelet concentrates make a difference.  As a patients or provider choose wisely.

June 29, 2011

Current Treatment of Chronic Pain is Lacking

Chronic pain is a significant problem which affects one in five patients according to the World Health Organization.

A recent article concludes that despite all the new treatments and improved understanding of the underlying pathology only 50% of the patients treated had a response to treatment and the reduction in pain was only about 30%.


Narcotics were the most commonly prescribed medication yet were associated with only small improvements in pain and were often misused.  Studies indicated that nearly 50% of long-term users may be misusing their narcotics thereby putting them at risk for overdose and death.

Regarding fusions of the lumbar spine it was noted that “High complication rates and repeat procedures are realities of spinal surgery ”

The Centeno-Schultz Clinic acknowledges the many of the main stream treatment modalities  for chronic pain are ineffective.  The Centeno-Schultz Clinic distinguishes itself by:

Publications in peer-reviewed journals.

Identifies the source of a patient’s pain as opposed to making it with oral narcotics.

Avoids  the use of NSAID’s.

Limits it use of steroids to low dose given their signficant complications including apoptosis.

Utilizes guided injections of regenerative therapies which include prolotherapy, PRP, platelet lysate(Regenexx PL), bone marrow derived and adipose derived stem cell therapy.

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