Stemcelldoc's Weblog

August 31, 2010

Critical Differences in PRP: Laboratory Prepared vs Bedside

Filed under: International Cellular Medicine Society — Tags: , — stemcelldoc @ 8:28 am

Not all platelet rich plasma (PRP) preparations are the same.   The majority of PRP preparations are created by bedside centrifuge units.  At Regenexx we are constantly striving to improve clinical results.  Our state of the art cellular laboratory enables us to experiment on different ways to get more platelets out of a given sample. 

We recently ran an experiment with 5 patient’s samples trying 5 different techniques to maximize platelet concentration in platelet rich plasma.  A flow cytometer was used to measure the number of platelets being isolated and how ‘clean” the isolation was in the mix..  A clean isolation was preferred since it meant it had little extra platelet matter( cell membranes.)

The results demonstrated that the centrifuge techniques similar to bedside PRP did NOT produce the best platelet concentrates.  In fact, they tended to produce less platelets and more non platelet matter than other techniques. 

A proprietary simple laboratory technique has been developed that provides more platelets and a much cleaner isolation.  It is called APC:  autologous platelet concentration so as to differentiate it from  PRP.  It produced 237% more platelets when compared with traditional bedside centrifuge units.

Below is a graph that demonstrates the significant differences.  The graph on the left has a wide mountain reflective of  significant non platelet matter whereas the graph on the right has a narrow tall peak reflective of a cleaner isolation.  Total number of platelets on the left is 200 vs 12oo produced by the laboratory.

Bottom line:  laboratory prepared autologous platelet concentrate provides a larger number of platelets in a cleaner isolation.  This difference translates to improved healing and faster recovery.

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